CARS Microspectrometer


Powerful and versatile tool for vibrational spectroscopy and chemically selective imaging:

  • Label-free optical imaging
  • Minimally invasive technique
  • Non-photobleaching signal
  • 3D sample imaging capability
  • NIR pump and Stokes wavelengths suitable for deep-tissue imaging
  • Picosecond pulse duration – good compromise between efficiency and spectral resolution

Coherent anti-Stokes Raman scattering (CARS) spectroscopy primarily was used in chemistry, physics and related fields. It is sensitive to the same vibrational signatures of molecules as seen in Raman spectroscopy, typically the nuclear vibrations of chemical bonds. Unlike Raman spectroscopy, CARS employs multiple photons to address the molecular vibrations, and produces a signal in which the emitted waves are coherent with one another. As a result, CARS is orders of magnitude stronger than spontaneous Raman emission. CARS is a third-order nonlinear optical process involving three laser beams: a pump beam of frequency ω pump , a Stokes beam of frequency ω Stokes and a probe beam at frequency ω probe . These beams interact with the sample and generate a coherent optical signal at the anti-Stokes frequency ω CARS = ω pump – ω Stokes + ω probe . The CARS signal ω CARS is resonantly enhanced when the difference between the pump ω pump and Stokes ω Stokes frequencies matches a vibrational transition ω vib of the molecule

Combining of coherent anti-Stokes Raman scattering (CARS) spectroscopy with the microscopy opens up unique method for chemical imaging. CARS microscopy permits vibrational imaging with high-sensitivity, high speed, and three-dimensional nearly diffraction limited spatial resolution.



  • Wide range of accessible vibrations: 740-4000 cm⁻¹
  • Minor fluorescence interference
  • High spectral resolution and sensitivity
  • Sub-wavelength spatial resolution
  • F-CARS, E-CARS, P-CARS detection geometries
  • Easy transformable to fluorescence, TPEF and SHG microscopes
  • Up to 1300 μm excitation for TPEF
  • Specially designed cost-effective picosecond tunable laser system

Related Applications

  • Species selective spectroscopy and microscopy
  • Multimodal nonlinear imaging
  • Deep tissue in vivo imaging
  • Long term live cell studies
  • Non-destructive research for the biological and material sciences
  • Your application is welcome…

Optical parameters
Spectral range 740-4000 cm-1
Pump/probe beams wavelength range 740-990 nm
Stokes wavelength 1064 nm
Spatial resolution 0.5 µm
Scanning system
Scanning method Sample movement
Scanning device Three-dimensional piezo stage
Maximum imaging speed 1) ~40 s
Travel range 100x100x20 µm
Resolution 1 nm
Pump laser
Model PT259-H
Pulse repetition rate 2) 1MHz
Tuning range 700-990,
1150-2300 nm
UV extension range (optional) 350-400 nm
Output power 3) 25 mW
UV range output power 4) 1 mW
Linewidth 3) < 8 cm -1
Typical pulse duration, ps 3) 5) ~5ps
Typical time bandwidth product < 0.8
Typical beam diameter 3) 6) 2 mm
Typical beam divergence 3) 7) < 2 mrad
M2 < 1.6

1) Measured 200 x 200 pixels.

2) Inquire for custom pulse repetition rates.

3) Measured at 800 nm.

4) Measured at 400 nm.

5) Pulse duration can vary depending on wavelength and pump energy.

6) Beam diameter at the FWHM level and can vary depending on the pump pulse energy.

7) Full angle measured at the FWHM level.



Fluorescence microscopy (available only with PT259 laser)
Excitation wavelength range 350-400 nm
Detection wavelength range 450-700 nm
TPEF Microscopy
Excitation wavelength range 700-1300 nm
Detection wavelength range 450-900 nm
SHG Microscopy
Excitation wavelength range 800-990, 1064 nm